Not Released
Sample In stock
Experience: Works
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Measurement

Part:BBa_K516130

Designed by: Susanna Zucca, Federica Sampietro, Giuseppe Bertoni, Paolo Magni   Group: iGEM11_UNIPV-Pavia   (2011-09-08)

Constitutive promoter [J23101] with mRFP, RBS B0030


Protein Generator regulated by BBa_J23101 constitutive promoter with BBa_B0030 high efficiency RBS, mRFP Reporter Coding Region and a double Transcription Terminators.
BBa_J23101 is the reference standard promoter for the computation of RPUs.
This measurement device belongs to a set of parts useful to estimate the RBS efficiency (BBa_K516131; BBa_K516132).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 619
    Illegal AgeI site found at 731
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/aConstitutive promoter [J23101] with mRFP, RBS B0030