Part:BBa_K5142043
Vaccinia virus A26L (partial)
This part includes the promoter and partial coding sequence (cds) of A26L, a membrane protein of vaccinia virus (VACV). Since A26L is located at the downstream region following A27L in vaccinia virus genome, we used partial A26L sequence (500 bp) as a homologous arm for the recombination to mutate A27L in our project.
The partial sequence of A26L was cloned by PCR amplification from the genome of vaccinia virus (GenBank ID: JX489136.1).
In our project, the A26L DNA sequence was served as a homologous arm for recombination only. Thus, the A26L sequence was not necessarily to be complete cds. Considering the whole A26L sequence is too long as a homologous arm, we chose 500 bp sequence adjacent to A27L only to construct the recombinant element.
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 523 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 524
Illegal PstI site found at 538
Illegal NotI site found at 7
Illegal NotI site found at 531 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 524 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 524
Illegal PstI site found at 538 - 1000COMPATIBLE WITH RFC[1000]
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