Coding

Part:BBa_K5142039

Designed by: Yuchen Liang   Group: iGEM24_HunanU   (2024-09-30)


Vaccinia virus A26L (partial)

This part includes the promoter and partial coding sequence (cds) of A26L, a membrane protein of vaccinia virus (VACV). Since A26L is located at the downstream region following A27L in vaccinia virus genome, we used partial A26L sequence (500 bp) as a homologous arm for the recombination to mutate A27L in our project.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 523
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 524
    Illegal PstI site found at 538
    Illegal NotI site found at 7
    Illegal NotI site found at 531
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 524
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 524
    Illegal PstI site found at 538
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None