Coding

Part:BBa_K5142038

Designed by: Yuchen Liang   Group: iGEM24_HunanU   (2024-09-30)


Reverse complementary GFP cds

This part contains the reverse complementary sequence of the coding sequence of green fluorescent protein (GFP). In our project, this part was inserted to the downstream of reverse poxviral H5 promoter (BBa_K5142012), and the expressed GFP can serve as an indicator of the successful transfection of the plasmid to cells infected by vaccinia virus and proximity of the plasmid to viral genome.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 740
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 741
    Illegal PstI site found at 755
    Illegal NotI site found at 7
    Illegal NotI site found at 748
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 215
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 741
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 741
    Illegal PstI site found at 755
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None