Plasmid_Backbone

Part:BBa_K514004

Designed by: Elena Moreno Martínez   Group: iGEM11_Sevilla   (2011-09-25)


GeneArt pMK-RQ (Kan)

A plasmid backbone in which GeneArt often sends the constructions they're asked for, with kanamicine resistance, and restriction sites for SfiI, NarI, DraII, NcoI, Eco57I, and Col E1 origin.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1994
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2308
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal PstI site found at 1994
    Illegal NotI site found at 9
    Illegal NotI site found at 2314
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2308
    Illegal BglII site found at 1626
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2308
    Illegal suffix found at 2
    Illegal PstI site found at 1994
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2308
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2323
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal PstI site found at 1994
    Illegal NgoMIV site found at 1848
    Illegal AgeI site found at 1955
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
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