Generator

Part:BBa_K511915

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)


Inducible rtTA-DD Transactivator Generator (UAS-Gal4-rtTA-DD) MammoBlock Device

This MammoBlock composite device produces the tetracycline-analogue-inducible and SHIELD-tunable rtTA-DD transactivator protein when induced with Gal4 transactivator variants.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 126
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 126
    Illegal NheI site found at 154
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 126
    Illegal XhoI site found at 83
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 126
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 126
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 258
    Illegal BsaI.rc site found at 897


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Parameters
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