Generator

Part:BBa_K511907

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)

Inducible Mnt-VP16 Transactivator Generator (TRE-Tight-Mnt-VP16) MammoBlock Device

This MammoBlock composite device produces the Mnt-VP16 transactivator protein when induced with tTA/rtTA transactivator variants in the presence of tetracycline analogues.


Mntvp16.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 445
    Illegal XbaI site found at 30
    Illegal PstI site found at 458
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 445
    Illegal PstI site found at 458
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 445
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 445
    Illegal XbaI site found at 30
    Illegal PstI site found at 458
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 445
    Illegal XbaI site found at 30
    Illegal PstI site found at 458
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aInducible Mnt-VP16 Transactivator Generator (TRE-Tight-Mnt-VP16) MammoBlock Device