Part:BBa_K511905

Repressible rtTA3 Transactivator Generator (Hef1a-LacO-rtTA3) MammoBlock Device

This MammoBlock composite device produces the rtTA3 repressor protein at a low, constitutive level that can be repressed by LacI variants.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1235
  Illegal PstI site found at 361
  Illegal PstI site found at 866
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1235
  Illegal PstI site found at 361
  Illegal PstI site found at 866
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1235
  Illegal BglII site found at 615
  Illegal XhoI site found at 1014
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1235
  Illegal PstI site found at 361
  Illegal PstI site found at 866
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1235
  Illegal PstI site found at 361
  Illegal PstI site found at 866
  Illegal NgoMIV site found at 749
  Illegal AgeI site found at 127
  
• 1000
  COMPATIBLE WITH RFC[1000]