Generator

Part:BBa_K511904

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)


Repressible LacI Repressor Generator (Hef1a-LacO-LacI) MammoBlock Device

This MammoBlock composite device produces the LacI repressor protein at a low, constitutive level that can be repressed by LacI variants. As a result, this device is expected to either limits its own production constant level or to oscillate; its behavior remains to be interrogated in future experiments.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 292
    Illegal PstI site found at 416
    Illegal PstI site found at 921
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 670
    Illegal BamHI site found at 255
    Illegal XhoI site found at 1069
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
    Illegal NgoMIV site found at 804
    Illegal AgeI site found at 80
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None