Part:BBa_K511824

Inducible Red Fluorescent Ligand Generator (UAS-Gal4-Delta-mCherry) MammoBlock Device

This MammoBlock composite device produces the red fluorescent juxtacrine signaling ligand Delta-mCherry when induced with Gal4 transactivator variants and otherwise produces Delta-mCherry at a low, basal (OFF) level.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 126
  Illegal PstI site found at 327
  Illegal PstI site found at 404
  Illegal PstI site found at 1247
  Illegal PstI site found at 1670
  Illegal PstI site found at 2347
  Illegal PstI site found at 2733
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 126
  Illegal NheI site found at 154
  Illegal PstI site found at 327
  Illegal PstI site found at 404
  Illegal PstI site found at 1247
  Illegal PstI site found at 1670
  Illegal PstI site found at 2347
  Illegal PstI site found at 2733
  Illegal NotI site found at 736
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 126
  Illegal XhoI site found at 83
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 126
  Illegal PstI site found at 327
  Illegal PstI site found at 404
  Illegal PstI site found at 1247
  Illegal PstI site found at 1670
  Illegal PstI site found at 2347
  Illegal PstI site found at 2733
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 126
  Illegal PstI site found at 327
  Illegal PstI site found at 404
  Illegal PstI site found at 1247
  Illegal PstI site found at 1670
  Illegal PstI site found at 2347
  Illegal PstI site found at 2733
  Illegal AgeI site found at 763
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 275
  Illegal BsaI site found at 715
  Illegal BsaI.rc site found at 1980
  Illegal BsaI.rc site found at 2152
  Illegal SapI site found at 1468
  Illegal SapI.rc site found at 2240