Generator
Part:BBa_K511817
Designed by: Grant Robinson Group: iGEM11_MIT (2011-09-28)
Inducible Yellow Fluorescent Protein Generator (TRE-Tight-EYFP-FF4x4) MammoBlock Device
This MammoBlock composite device produces the yellow fluorescent protein EYFP-FF4x4 when induced with tTA/rtTA transactivator variants in the presence of tetracycline analogues, and otherwise produces EYFP-FF4x4 at a low, basal (OFF) level. Note that repression of the EYFP-4xFF4 protein can be mediated by FF4 micro RNA.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal XbaI site found at 30
Illegal PstI site found at 557 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal PstI site found at 557 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal XbaI site found at 30
Illegal PstI site found at 557 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal XbaI site found at 30
Illegal PstI site found at 557 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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