Generator

Part:BBa_K511817

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)


Inducible Yellow Fluorescent Protein Generator (TRE-Tight-EYFP-FF4x4) MammoBlock Device

This MammoBlock composite device produces the yellow fluorescent protein EYFP-FF4x4 when induced with tTA/rtTA transactivator variants in the presence of tetracycline analogues, and otherwise produces EYFP-FF4x4 at a low, basal (OFF) level. Note that repression of the EYFP-4xFF4 protein can be mediated by FF4 micro RNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal PstI site found at 557
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal PstI site found at 557
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal PstI site found at 557
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal PstI site found at 557
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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