Generator

Part:BBa_K511812

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)

CI434-VP16-Inducible Red Fluorescence Generator (minCMV-1xCI434-mKate) MammoBlock Device

This MammoBlock composite device produces the monomeric red fluorescent protein mKate driven by the minCMV-1xCI434 promoter. In the absence of CI434-VP16, transcription of mKate is very low, but in the presence of CI434-VP16, transcription is moderately high.

C1434.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 769
    Illegal SapI.rc site found at 151


[edit]
Categories
Parameters
n/aCI434-VP16-Inducible Red Fluorescence Generator (minCMV-1xCI434-mKate) MammoBlock Device