Signalling

Part:BBa_K511810

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)


Repressible Tango Receptor Generator (Hef1a-LacO-AVPR2-TEVs-Gal4-VP16) MammoBlock Device

This MammoBlock composite device produces the AVPR2-TEVs-Gal4-VP16 synthetic fusion receptor adapted from the proprietary Tango system. This device is designed to activate expression from UAS-Gal4-driven elements in the presence of vasopressin and the B-Arrestin-2-TEV-Protease construct. Transcription of the AVPR2-TEVs-Gal4-VP16 receptor is driven by the Hef1a-LacO promoter and therefore repressible by LacI variants.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
    Illegal PstI site found at 1649
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 292
    Illegal NheI site found at 1402
    Illegal PstI site found at 416
    Illegal PstI site found at 921
    Illegal PstI site found at 1649
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 670
    Illegal BamHI site found at 255
    Illegal BamHI site found at 2411
    Illegal BamHI site found at 2441
    Illegal XhoI site found at 1069
    Illegal XhoI site found at 2907
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
    Illegal PstI site found at 1649
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
    Illegal PstI site found at 1649
    Illegal NgoMIV site found at 804
    Illegal NgoMIV site found at 1494
    Illegal AgeI site found at 80
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2826
    Illegal SapI site found at 1819


[edit]
Categories
Parameters
n/aRepressible Tango Receptor Generator (Hef1a-LacO-AVPR2-TEVs-Gal4-VP16) MammoBlock Device