Signalling

Part:BBa_K511809

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)


Repressible Tango Ligand Generator (Hef1a-LacO-pDisplay-Vasopressin-MYC) MammoBlock Device

This MammoBlock composite device produces the pDisplay-Vasopressin-MYC synthetic ligand designed to activate expression from the AVPR2-TEVs-Gal4-VP16 signaling receptor. Because this ligand is displayed in a membrane-bound, extracellular fashion, it is ideal for juxtacrine signaling that is orthogonal to other juxtacrine signaling systems, particularly the Notch-Delta system. Transcription is driven by the Hef1a-LacO promoter and therefore repressible by LacI variants.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 292
    Illegal PstI site found at 416
    Illegal PstI site found at 921
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 670
    Illegal BamHI site found at 255
    Illegal XhoI site found at 1069
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
    Illegal NgoMIV site found at 804
    Illegal AgeI site found at 80
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aRepressible Tango Ligand Generator (Hef1a-LacO-pDisplay-Vasopressin-MYC) MammoBlock Device