Part:BBa_K511806

Repressible Red Fluorescent Ligand Generator (Hef1a-LacO-Delta-mCherry) MammoBlock Device

This MammoBlock composite device produces the red fluorescent juxtacrine signaling ligand Delta-mCherry at a low, constitutive level and can be repressed by LacI variants.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 416
  Illegal PstI site found at 921
  Illegal PstI site found at 1382
  Illegal PstI site found at 1459
  Illegal PstI site found at 2302
  Illegal PstI site found at 2725
  Illegal PstI site found at 3402
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 292
  Illegal PstI site found at 416
  Illegal PstI site found at 921
  Illegal PstI site found at 1382
  Illegal PstI site found at 1459
  Illegal PstI site found at 2302
  Illegal PstI site found at 2725
  Illegal PstI site found at 3402
  Illegal NotI site found at 1791
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 670
  Illegal BamHI site found at 255
  Illegal XhoI site found at 1069
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 416
  Illegal PstI site found at 921
  Illegal PstI site found at 1382
  Illegal PstI site found at 1459
  Illegal PstI site found at 2302
  Illegal PstI site found at 2725
  Illegal PstI site found at 3402
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 416
  Illegal PstI site found at 921
  Illegal PstI site found at 1382
  Illegal PstI site found at 1459
  Illegal PstI site found at 2302
  Illegal PstI site found at 2725
  Illegal PstI site found at 3402
  Illegal NgoMIV site found at 804
  Illegal AgeI site found at 80
  Illegal AgeI site found at 1818
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1330
  Illegal BsaI site found at 1770
  Illegal BsaI.rc site found at 3035
  Illegal BsaI.rc site found at 3207
  Illegal SapI site found at 2523
  Illegal SapI.rc site found at 3295