Plasmid

Part:BBa_K5116018

Designed by: Zhe Jing Yourong Yan   Group: iGEM24_HNU-China   (2024-10-02)


pGADT7-CYC1-B4GalT1

A plasmid that expresses B4GalT1，which codes a enzyme that can transfer a glycosidic acid(usually glucose uridine diphosphate or galactose urdine diphosphate) to a molecule.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1989
    Illegal XbaI site found at 8417
    Illegal PstI site found at 8
    Illegal PstI site found at 2035
    Illegal PstI site found at 4505
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1989
    Illegal NheI site found at 4495
    Illegal NheI site found at 7985
    Illegal PstI site found at 8
    Illegal PstI site found at 2035
    Illegal PstI site found at 4505
    Illegal NotI site found at 4516
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1989
    Illegal BglII site found at 1897
    Illegal BamHI site found at 2017
    Illegal XhoI site found at 2029
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1989
    Illegal XbaI site found at 8417
    Illegal PstI site found at 8
    Illegal PstI site found at 2035
    Illegal PstI site found at 4505
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1989
    Illegal XbaI site found at 8417
    Illegal PstI site found at 8
    Illegal PstI site found at 2035
    Illegal PstI site found at 4505
    Illegal NgoMIV site found at 210
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 860
    Illegal BsaI.rc site found at 5713
    Illegal SapI site found at 4630


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Categories
Parameters
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