Signalling

Part:BBa_K511400

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-27)


AVPR2-TEVs-Gal4-VP16 Signaling Receptor MammoBlock

This part encodes a fusion protein consisting of the AVPR2 receptor, the recognition sequence for the tobacco etch virus (TEV) protease, and the Gal4-VP16 transactivator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 105
    Illegal PstI site found at 352
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1114
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1610
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
    Illegal NgoMIV site found at 197
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1529
    Illegal SapI site found at 522


[edit]
Categories
Parameters
n/aAVPR2-TEVs-Gal4-VP16 Signaling Receptor MammoBlock