Coding

Part:BBa_K511207

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-27)

Gal4-VP16 Transactivator MammoBlock

This part encodes a transactivator protein that binds to the Gal4 galactose upstream activation sequence. The part is a fusion between the Gal4 DNA-binding domain and the viral VP16 transactivation domain. Gal4-VP16 has been proven to be a particularly powerful transactivator in mammalian cells, and is well suited to situations where levels of high transcriptional activation are necessary.

For more information, consult the MIT iGEM 2011 wiki here.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 461
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 380


[edit]
Categories
Parameters
n/aGal4-VP16 Transactivator MammoBlock