Part:BBa_K511206

LacI-miRFF4 Repressor MammoBlock

This part encodes a dual transcriptional/translational repression system including a protein that binds tightly to both the lactose operator and an intronic micro RNA (miRNA) that binds tightly to the FF4 sequence. LacI-miRFF4 is best used with an FF4-tagged protein that is transcribed by a promoter that contains a lactose operator, allowing for combined repression at a transcriptional and translational level. Because transcriptional repression from LacI and miRNA-mediated degradation of FF4-tagged mRNA are relieved once the respective elements have been degraded or diluted in a cell, the LacI-miRFF4 construct is particularly well-suited to circuits that require strong but transient repression, such as a fast toggle switch.

For more information, consult the MIT iGEM 2011 wiki here.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1332
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1332
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1332
  Illegal XhoI site found at 1229
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1332
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1332
  
• 1000
  COMPATIBLE WITH RFC[1000]