Part:BBa_K511205

LacI-KRAB Repressor MammoBlock

This part encodes a repressor protein that binds tightly to the lactose operator originating from Escherichia coli. This protein is a fusion between the LacI repressor and the Kruppel-associated box (KRAB), a domain previously shown to strongly repress transcription from affected promoters via chromatin silencing. This repressor has been codon-optimized for mammalian expression and is capable of exerting very strong repression on promoters with internal lactose operator sites. Because LacI-KRAB-mediated repression can last for days due to its chromatin remodeling activity, LacI-KRAB is recommended for situations in which a change in transcriptional activity must be stable for extended periods of time, such as a permanent switch.

For more information, consult the MIT iGEM 2011 wiki here.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1417
  Illegal SapI.rc site found at 1381