Coding

Part:BBa_K511204

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-27)

LacI Repressor MammoBlock

This part encodes a repressor protein that binds tightly to the lactose operator originating from Escherichia coli. This protein has been codon-optimized for mammalian expression and is capable of exerting moderate repression on promoters with internal lactose operator sites.

Figures 1 and 2 below show typical levels of repression mediated by the LacI repressor.

For more information, consult the MIT iGEM 2011 wiki here.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aLacI Repressor MammoBlock