Coding
Part:BBa_K511203
Designed by: Grant Robinson Group: iGEM11_MIT (2011-09-27)
rtTA3 Tetracycline-Inducible Transactivator MammoBlock
This part encodes a transactivator composed of the DNA-binding domain for the tetracycline operator sequence and the viral VP16 transactivation domain; this protein has been engineered over previous versions to be more sensitive to tetracycline and its analogues.
Figures 1 and 2 below show expression from a promoter with six tetracycline response elements (TRE-Tight) in the presence of rtTA3 and various levels of doxycycline, a tetracycline analogue.
For more information, consult the MIT iGEM 2011 wiki here.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
n/a | rtTA3 Tetracycline-Inducible Transactivator MammoBlock |