Coding

Part:BBa_K511202

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-25)

rtTA-DD Transactivator MammoBlock

This part encodes a protein that combines the rtTA transactivator with the DD destabilization domain. The DD domain causes attached proteins to be degraded extremely quickly in mammalian cells; however, the DD domain is stable in the presence of the SHIELD molecule, allowing for tunable protein expression. Because the rtTA transactivator is dependent upon doxycycline for activation, transactivation by rtTA-DD is unique among transactivators for the dynamic range of expression it can activate.

For more information, consult the MIT iGEM 2011 wiki here.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 16
    Illegal BsaI.rc site found at 655


[edit]
Categories
Parameters
n/artTA-DD Transactivator MammoBlock