Coding

Part:BBa_K511201

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-25)

Mnt-VP16 Transactivator MammoBlock

This part encodes a protein that combines the Mnt DNA-binding domain with the VP16 viral transactivation domain. This synthetic transactivator is able to moderately activate expression from minCMV-Mnt promoter variants.


For more information, consult the MIT iGEM 2011 wiki here.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 93
    Illegal PstI site found at 106
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 93
    Illegal PstI site found at 106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 93
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 93
    Illegal PstI site found at 106
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 93
    Illegal PstI site found at 106
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aMnt-VP16 Transactivator MammoBlock