Reporter

Part:BBa_K511103

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)

amCyan-miRFF4 MammoBlock

This part is an engineered variant of the original green fluorescent protein from Aequorea victoria that fluoresces cyan. Additionally, this protein contains an intronic micro RNA (miRNA) that binds tightly to the FF4 sequence. This protein is therefore a dual repressor/reporter that typically serves to indicate which cells have miRFF4 in mammalian experiments.

For more information, consult the MIT iGEM 2011 wiki here.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 329
    Illegal PstI site found at 881
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 329
    Illegal PstI site found at 881
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 329
    Illegal XhoI site found at 226
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 329
    Illegal PstI site found at 881
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 329
    Illegal PstI site found at 881
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aamCyan-miRFF4 MammoBlock