Reporter

Part:BBa_K511102

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-25)

mKate MammoBlock

A mammalian-codon optimized red fluorescent protein. This protein is a significantly engineered reporter protein that, unlikely many fluorescent proteins, is monomeric and therefore ideal for fusion constructs.

Figures 1 and 2 show typical levels of mKate-mediated fluorescence following transfection of HEK-293 cells with mKate driven by the Hef1a low-level constitutive promoter.

For more information, consult the MIT iGEM 2011 wiki here.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 634
    Illegal SapI.rc site found at 16


[edit]
Categories
Parameters
n/amKate MammoBlock