Regulatory

Part:BBa_K511004

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)

TRE-Tight-LacOid Promoter MammoBlock

This part encodes an inducible promoter that can be activated by tTA/rtTA transactivator variants in the presence of tetracycline analogues and can be repressed by variants of the LacI transcriptional repressor.

For more information, consult the MIT iGEM 2011 wiki here.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 34
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 34
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 34
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aTRE-Tight-LacOid Promoter MammoBlock