Regulatory

Part:BBa_K511001

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-25)

minCMV-1xCI434 Promoter MammoBlock

This part encodes a promoter that is moderately inducible by the CI434-VP16 transactivator and off otherwise.

As shown in Figures 1 and 2 below, induction with the CI434-VP16 transactivator results in marked increase in fluorescence from a construct containing a red fluorescent protein (mKate) driven by the minCMV-1xCI434 promoter.

For more information, consult the MIT iGEM 2011 wiki here.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aminCMV-1xCI434 Promoter MammoBlock