Coding

Part:BBa_K510033

Designed by: Paola Gallardo, Rafael R. Daga   Group: iGEM11_UPO-Sevilla   (2011-09-20)

tetR-CSD

It is composed by tetracycline repressor from Escherichia coli and the chromoshadow domain of nucleosomal histone binding protein, Swi6, from the fission yeast Schizosaccharomyces pombe. TetR is responsible for binding to tetR operator sites, heading engineering silencing proteins to regulatory sequences. CSD is a protein-protein interaction domain that contains a dimerization motif that creates a cleft, mediating transcription and epigenetic regulation in the nucleus, by heterochromatin formation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 645
    Illegal BamHI site found at 622
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None