Regulatory

Part:BBa_K510032

Designed by: Paola Gallardo, Rafael R. Daga   Group: iGEM11_UPO-Sevilla   (2011-09-20)

urg1 promoter

Uracil-regulable urg1 promoter, that allows tight expression control of ectopic genes with otherwise minimal side effects on genome-wide gene expression. Transcription is activated after 5 minutes of uracil addition and peaks within 30 minutes. Similarly, transcription rapidly drops after transfer to medium without uracil. It is used in yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 446
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal XhoI site found at 306
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None