Regulatory
Part:BBa_K510032
Designed by: Paola Gallardo, Rafael R. Daga Group: iGEM11_UPO-Sevilla (2011-09-20)
urg1 promoter
Uracil-regulable urg1 promoter, that allows tight expression control of ectopic genes with otherwise minimal side effects on genome-wide gene expression. Transcription is activated after 5 minutes of uracil addition and peaks within 30 minutes. Similarly, transcription rapidly drops after transfer to medium without uracil. It is used in yeast.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 446
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
Illegal XhoI site found at 306 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |