Composite

Part:BBa_K510031

Designed by: Paola Gallardo, Rafael R. Daga   Group: iGEM11_UPO-Sevilla   (2011-09-20)

GFP-Tadh1- tetO4- Tact1

This composite part is formed by four main elements: GFP, adh1 terminator, tetO4 and act1 terminator. Coding sequence of GFP reporter protein is followed by the transcriptional terminator of ADH1 gene. The next part is denominated tetO4, that consist of four 19 nucleotices tetR operator sequences, alternated with spacers. And the last part is the Act1 transcriptional terminator that function as insulator. This part should be interted behind a promoter. In association with Tadh1-tetO2 part, it functions as a transcription regulator, by compacting intermediate sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1262
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1262
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1262
    Illegal BamHI site found at 627
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1262
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1262
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None