Reporter

Part:BBa_K509005

Designed by: Ben Jevans   Group: iGEM11_UEA-JIC_Norwich   (2011-09-19)

GLuc

Usage and Biology

This is a Bioluminescent luciferase protein that emits green light, and so can be used as a reporter. It was isolated from the sea creature Gaussia princeps. This version of the gene has been optimised for use in the algal species Chlamydomonas reinhardtii.


Improvement from SUSTech_Shenzhen 2019

We designed

Biobrick BBa_K2086004, hGluc

as a improvement for the Gluc to the humanized Gaussia princeps which can be expressed in human cell lines.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 205
    Illegal NgoMIV site found at 550
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None