Composite

Part:BBa_K5048075

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-24)


araBAD promoter-LAP-GST

Solubility-enhanced LAP-GST regulated by araBAD promoter to optimize the expression of the protein. See more in BBa_K5048066.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1762
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1762
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2805
    Illegal BglII site found at 2903
    Illegal BamHI site found at 239
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1762
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1762
    Illegal NgoMIV site found at 1011
    Illegal NgoMIV site found at 2277
    Illegal AgeI site found at 74
    Illegal AgeI site found at 643
    Illegal AgeI site found at 2299
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56
    Illegal SapI site found at 2625


Design

We used homologous recombination to construct this part, so we need to design proper homologous arms. However, when we encounter the situation that two segments come from the same kind of backbone, we need to pay extra attention to the specificity of the primers.


Source

See our parts: BBa_K5048016, BBa_K5048064,BBa_K5048066, BBa_B0015


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Categories
Parameters
None