Composite

Part:BBa_K5048073

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-24)


T7 promoter-SUMO-HSP60-FLAG

HSP60 protein with a SUMO tag on the N-terminus and a FLAG tag on the C-terminus. Solubility enhanced to test the interaction with LAP. See more in BBa_K5048065.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 51
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2100
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3866
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 51
    Illegal NgoMIV site found at 2441
    Illegal AgeI site found at 2596
    Illegal AgeI site found at 3074
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1338


Design

SUMO is an N-terminus tag. When we introduced it into the HSP60 gene sequence, we had to introduce the start codon.


Source

See our parts: BBa_I719005 ,BBa_K5048065


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Categories
Parameters
None