Composite

Part:BBa_K5048072

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-24)

T7 promoter-Neae-HSP60-FLAG

Codes for one of our team’s fusion proteins, presenting the HSP60 protein on the surface of E.coli BL21(DE3) to enable the bacteria-intestine adhesion and the bacteria-bacteria adhesion. Codon optimized for expression in E.coli. See more in BBa_K5048069.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 51
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 92
    Illegal XhoI site found at 3872
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 51
    Illegal NgoMIV site found at 2447
    Illegal AgeI site found at 2602
    Illegal AgeI site found at 3080
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1344
[edit]
Categories
Parameters
None