Composite

Part:BBa_K5048071

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-24)

T7 promoter-Neae-LAP-6xHis

Codes for one of our team’s fusion proteins, presenting the LAP protein on the surface of E.coli BL21(DE3) to enable the bacteria-intestine adhesion and the bacteria-bacteria adhesion. Codon optimized for expression in E.coli. See more in BBa_K5048068


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 51
    Illegal PstI site found at 3613
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3613
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4656
    Illegal BglII site found at 4754
    Illegal BamHI site found at 92
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 51
    Illegal PstI site found at 3613
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 51
    Illegal PstI site found at 3613
    Illegal NgoMIV site found at 2862
    Illegal NgoMIV site found at 4128
    Illegal AgeI site found at 2494
    Illegal AgeI site found at 4150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1344
    Illegal SapI site found at 4476
[edit]
Categories
Parameters
None