Composite

Part:BBa_K5048067

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-24)


T7 promoter-HSP60-FLAG

HSP60 protein with a FLAG tag on the C-terminus. Used to test the expression of HSP60.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 51
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 92
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 51
    Illegal NgoMIV site found at 433
    Illegal AgeI site found at 588
    Illegal AgeI site found at 1066
  • 1000
    COMPATIBLE WITH RFC[1000]


Design

HSP60 protein with a FLAG tag on the C-terminus and a T7 promoter to ensure the expression of the protein.Used to test the expression of HSP60.


Source

See our parts: BBa_K5048047, BBa_K5048060


Experiments

See more in BBa_K5048060


[edit]
Categories
Parameters
None