Part:BBa_K5048059

HSP60

HSP60(heat shock protein 60) codes for the membrane protein of small intestinal epithelial cells, which can adhere to LAP.

Design Notes

HSP60 is a protein expressed on the cell membrane of the human intestine epithelia and can bind to LAP.

Source

HSPD1 heat shock protein family D (Hsp60) member 1 [Homo sapiens (human)] - Gene - NCBI (nih.gov) CCD：CCDS Report for Consensus CDS (nih.gov)

Expriments

We handed this original sequence to the biology company Tsingke and implemented condon optimization. To facilitate the detection of protein products, we added a FLAG tag at the C-terminal of the protein forming HSP60-FLAG(BBa_K5048059) and inserted this improved sequence into pET-28a backbone to create the plasmid HSP60-FLAG.

Applications of BBa_K5048059

We use this part as an adhesin to achieve cell-cell adhesion.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 712
  Illegal SpeI site found at 1144
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 712
  Illegal SpeI site found at 1144
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 712
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 712
  Illegal SpeI site found at 1144
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 712
  Illegal SpeI site found at 1144
  Illegal AgeI site found at 36
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 33