Plasmid

Part:BBa_K5048056

Designed by: Yiheng Liao   Group: iGEM24_WHU-China   (2024-09-22)


plasmid pBAD lasR

This plasmid uses pBAD as backbone. Upon induction with arabinose, the bacteria will express lasR. On this basis, with the addition of lasI, the bacteria will express the ccdB toxin and die.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1456
    Illegal PstI site found at 1436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1456
    Illegal PstI site found at 1436
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1456
    Illegal BglII site found at 1432
    Illegal BamHI site found at 1422
    Illegal XhoI site found at 1428
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1456
    Illegal PstI site found at 1436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1456
    Illegal PstI site found at 1436
    Illegal NgoMIV site found at 322
    Illegal AgeI site found at 519
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1194
    Illegal BsaI site found at 1635
    Illegal BsaI site found at 2702
    Illegal SapI.rc site found at 3784


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Parameters
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