Coding

Part:BBa_K5048045

Designed by: Yiheng Liao   Group: iGEM24_WHU-China   (2024-09-22)


ccdB-dt-Plux-ccdA-dt-J23106-LuxR-dt

This fragment will work together with the araBAD promoter on the pBAD backbone. When not induced, the bacteria survive. Upon induction with arabinose, the bacteria express the ccdB toxin and die. On this basis, with the addition of luxI induction, the bacteria will express the ccdA antitoxin and survive.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 897
    Illegal NheI site found at 920
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216


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Categories
Parameters
None