Plasmid

Part:BBa_K5048044

Designed by: Yiheng Liao   Group: iGEM24_WHU-China   (2024-09-22)


plasmid luxR

This plasmid uses pET-28a as backbone.T7 promoter regulates the expression of luxR, which would bind to LuxI and induce Plux to express deGFP as signal.The insertion fragment is complete synthesis . The backbone is purchased from Genscript.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6543
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1716
    Illegal NgoMIV site found at 4763
    Illegal NgoMIV site found at 4923
    Illegal NgoMIV site found at 6511
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3842


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Categories
Parameters
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