Plasmid

Part:BBa_K5048043

Designed by: Yiheng Liao   Group: iGEM24_WHU-China   (2024-09-22)


plasmid lasR

We used pET-28a as a backbone. T7 promoter regulates the expression of lasR, which would bind to LasI and induce Plas to express deGFP as signal.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6545
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 351
    Illegal NgoMIV site found at 1718
    Illegal NgoMIV site found at 4765
    Illegal NgoMIV site found at 4925
    Illegal NgoMIV site found at 6513
    Illegal AgeI site found at 548
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3844


[edit]
Categories
Parameters
None