Coding

Part:BBa_K5048040

Designed by: Yiheng Liao   Group: iGEM24_WHU-China   (2024-09-22)


T7 promoter(lac repressible)-lasR-Plas-deGFP

T7 promoter regulates the expression of lasR, which would bind to LasI and induce Plas to express deGFP as signal.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 351
    Illegal AgeI site found at 548
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None