Plasmid

Part:BBa_K5048026

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-14)


plasmid PBAD_AQ_GST

This plasmid uses pBAD_hisA as backbone. This part is the surface-decorating system which display small peptides AQ in the outer leaflet of the outer-membrane of E.coli. The DDDDK enterokinase cleavage site can be recognized by enteroskinase and small peptides will be cut off. It contains the araBAD promoter, lpp'OmpA(codon optimised), enterokinase site,AQ, GST tag, rrnB T1 terminator and T7Te terminator.It is used in the verification of the enzymatic cleavage system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1210
    Illegal XbaI site found at 323
    Illegal PstI site found at 1190
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1210
    Illegal PstI site found at 1190
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1210
    Illegal BglII site found at 1186
    Illegal BamHI site found at 239
    Illegal BamHI site found at 1176
    Illegal XhoI site found at 1182
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1210
    Illegal XbaI site found at 323
    Illegal PstI site found at 1190
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1210
    Illegal XbaI site found at 323
    Illegal PstI site found at 1190
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1389
    Illegal BsaI site found at 2456
    Illegal SapI site found at 56
    Illegal SapI.rc site found at 3538


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Categories
Parameters
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