Plasmid

Part:BBa_K5048025

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-14)


plasmid PBAD_QEP_GST

This plasmid uses pBAD_hisA as backbone. This part is the surface-decorating system which display small peptides QEP in the outer leaflet of the outer-membrane of E.coli. The DDDDK enterokinase cleavage site can be recognized by enteroskinase and small peptides will be cut off. It contains the araBAD promoter, lpp'OmpA(codon optimised), enterokinase site, QEP, GST tag, rrnB T1 terminator and T7Te terminator.It is used in the verification of the enzymatic cleavage system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1216
    Illegal XbaI site found at 323
    Illegal PstI site found at 1196
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1216
    Illegal PstI site found at 1196
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1216
    Illegal BglII site found at 1192
    Illegal BamHI site found at 239
    Illegal BamHI site found at 1182
    Illegal XhoI site found at 1188
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1216
    Illegal XbaI site found at 323
    Illegal PstI site found at 1196
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1216
    Illegal XbaI site found at 323
    Illegal PstI site found at 1196
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1395
    Illegal BsaI site found at 2462
    Illegal SapI site found at 56
    Illegal SapI.rc site found at 472
    Illegal SapI.rc site found at 3544


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Categories
Parameters
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