Plasmid

Part:BBa_K5048024

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-14)


plasmid AQ_6xhis

This plasmid uses pET-28a as backbone. This part is the surface-decorating system which display small peptide AQ in the outer leaflet of the outer-membrane of E.coli. The DDDDK enterokinase cleavage site can be recognized by enteroskinase and small peptides will be cut off. It contains the T7 promoter, Lac operator, lpp'OmpA(codon optimised), enterokinase site, AQ, 6xhis tag, rrnB T1 terminator and T7Te terminator.It is used in the verification of the enzymatic cleavage system


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5665
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5665
    Illegal NotI site found at 5690
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5665
    Illegal BglII site found at 4964
    Illegal BamHI site found at 5659
    Illegal XhoI site found at 5699
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5665
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5665
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3184
    Illegal NgoMIV site found at 3344
    Illegal NgoMIV site found at 4932
    Illegal AgeI site found at 5409
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2263


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Categories
Parameters
None