Plasmid

Part:BBa_K5048020

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-13)


plasmid Lpp'OmpA-lptE

This plasmid uses pET-28a as backbone.This part is the surface-decorating system which display lipoprotein lptE in the outer leaflet of outer-membrane in E.coli.It contains the T7 promoter, Lac operator, Lpp'OmpA, lptE(sp removed), FLAG tag, rrnB T1 terminator and T7Te terminator.It is used in the verification of the surface display system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6160
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6160
    Illegal NotI site found at 6185
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6160
    Illegal BglII site found at 4964
    Illegal BamHI site found at 6154
    Illegal XhoI site found at 6194
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6160
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6160
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3184
    Illegal NgoMIV site found at 3344
    Illegal NgoMIV site found at 4932
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2263


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Categories
Parameters
None