Composite

Part:BBa_K5048012

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-13)


T7-lac-Lpp'OmpA(codon optimised)-DDDDK-QEP-GST-dt

This part is the surface-decorating system which display small peptides QEP in the outer leaflet of the outer-membrane of E.coli. The DDDDK enterokinase cleavage site can be recognized by enteroskinase and small peptides will be cut off. It contains the T7 promoter, Lac operator, Lpp'OmpA(codon optimised), enterokinase site, QEP, GST tag, rrnB T1 terminator and T7Te terminator.It is used in the verification of the enzymatic cleavage system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 426
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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