Coding

Part:BBa_K5048010

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-12)


DDDDK_AQ_6xhis

DDDDK is the enterokinase cleavage site. AQ is the short peptide composed of alanine and glutamine. They are fused with 6xhis tag for further investigation. All codons are optimised for E.coli. It is used in our surface display system for enzymatic cleavage verification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None