Composite

Part:BBa_K5048002

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-10)


T7-lac-Lpp'OmpA-lptE-FLAG-double terminator

This part is the surface-decorating system which display lipoprotein lptE in the outer leaflet of outer-membrane in E.coli.It contains the T7 promoter, Lac operator, Lpp'OmpA, lptE(sp removed), FLAG tag, rrnB T1 terminator and T7Te terminator.It is used in the verification of the surface display system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None